the empty pgl3 basic vector was used as the negative control (Promega)
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the empty pgl3 basic vector was used as the negative control
The Empty Pgl3 Basic Vector Was Used As The Negative Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+empty+pgl3+basic+vector+was+used+as+the+negative+control/pgl3+basic/pmc06607394-115-2-17
Average 90 stars, based on 1 article reviews
The Empty Pgl3 Basic Vector Was Used As The Negative Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+empty+pgl3+basic+vector+was+used+as+the+negative+control/pgl3+basic/pmc06607394-115-2-17
Average 90 stars, based on 1 article reviews
the empty pgl3 basic vector was used as the negative control - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Significant association of EED promoter hypomethylation with colorectal cancer"
Article Title: Significant association of EED promoter hypomethylation with colorectal cancer
Journal: Oncology Letters
doi: 10.3892/ol.2019.10432
Figure Legend Snippet: Dual-luciferase reporter assay in the 293T cell line. The pGL3-Basic and pGL3-Promoter vectors were used as negative and positive controls, respectively. The relative luciferase level is displayed for pGL3-Promoter, pGL3-Basic and pGL3-EED The pGL3-EED indicates the recombinant EED fragment ligated to the pGL3-Basic vector. An analysis of variance and Bonferroni's correction were applied for statistical analysis. P<0.05 was considered to indicate a statistically significant difference. *P<0.0001. EED, embryonic ectoderm development; Luc, luciferase.
Techniques Used: Luciferase, Reporter Assay, Recombinant, Plasmid Preparation
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Plasmid Preparation:Article Title: Significant association of EED promoter hypomethylation with colorectal cancer Article Snippet: The fragment was then purified by Cycle Pure kit (Omega Bio-Tek, Inc., Norcross, GA, USA) and the target DNA fragment was cloned into a pGL3 vector (Promega Cooperation, Madison, WI, USA) using DNA Ligation kit (Takara Bio, Inc., Otsu, Japan). .. The empty Negative Control:Article Title: Significant association of EED promoter hypomethylation with colorectal cancer Article Snippet: The fragment was then purified by Cycle Pure kit (Omega Bio-Tek, Inc., Norcross, GA, USA) and the target DNA fragment was cloned into a pGL3 vector (Promega Cooperation, Madison, WI, USA) using DNA Ligation kit (Takara Bio, Inc., Otsu, Japan). .. The empty Positive Control:Article Title: Significant association of EED promoter hypomethylation with colorectal cancer Article Snippet: The fragment was then purified by Cycle Pure kit (Omega Bio-Tek, Inc., Norcross, GA, USA) and the target DNA fragment was cloned into a pGL3 vector (Promega Cooperation, Madison, WI, USA) using DNA Ligation kit (Takara Bio, Inc., Otsu, Japan). .. The empty Luciferase:Article Title: Significant association of EED promoter hypomethylation with colorectal cancer Article Snippet: The fragment was then purified by Cycle Pure kit (Omega Bio-Tek, Inc., Norcross, GA, USA) and the target DNA fragment was cloned into a pGL3 vector (Promega Cooperation, Madison, WI, USA) using DNA Ligation kit (Takara Bio, Inc., Otsu, Japan). .. The empty |
